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Sulfo-Cyanine5 NHS ester – CAS 2230212-27-6

Characterisation

CAS: 2230212-27-6

IUPAC Name: sodium;1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate

Other names: Sulfo-Cyanine5 NHS Ester, F03-0032, diSulfo-Cy5 NHS Ester

Molecular weight: 761.8 g/mol

Molecular formula: C₃₆H₄₀N₃NaO₁₀S₂

InChI: InChI=1S/C36H41N3O10S2.Na/c1-35(2)26-22-24(50(43,44)45)15-17-28(26)37(5)30(35)12-8-6-9-13-31-36(3,4)27-23-25(51(46,47)48)16-18-29(27)38(31)21-11-7-10-14-34(42)49-39-32(40)19-20-33(39)41;/h6,8-9,12-13,15-18,22-23H,7,10-11,14,19-21H2,1-5H3,(H-,43,44,45,46,47,48);/q;+1/p-1

Solubility: Water, DMF, DMSO

Excitation/absorption maximum: 646 nm

Emission maximum: 662 nm

Description

Introducing our water-soluble Sulfo-Cyanine5 NHS ester, a counterpart to Cy5® NHS ester, designed for seamless labeling of diverse amine-containing molecules in an aqueous environment, eliminating the need for any organic co-solvent. This innovation proves especially valuable for labeling proteins prone to denaturation in the presence of organic co-solvents or those with low solubility.

Sulfo-Cyanine5 stands as an analog of Cy5®, a highly popular fluorophore, ensuring compatibility with various equipment such as plate readers, microscopes, and imagers. Its exceptional hydrophilicity and water solubility make it an ideal choice for applications where other dyes may fall short.

Discover a versatile replacement for Cy5® and DyLight 649, as our sulfo-Cyanine 5 opens doors to a wide array of applications without compromising performance. Elevate your experiments with a dye that transcends limitations and delivers reliable results in diverse research settings.

References

• Shukla S, Wang C, Beiss V, Steinmetz NF. Antibody Response against Cowpea Mosaic Viral Nanoparticles Improves In Situ Vaccine Efficacy in Ovarian Cancer. ACS Nano. 2020;14(3):2994-3003. doi:10.1021/acsnano.9b07865

 

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